A hyperbranched β-d-glucan with compact coil conformation from Lignosus rhinocerotis sclerotia. (15th June 2017)

• Record Type: Journal Article
• Authors: Hu, Ting; Huang, Qilin; Wong, Kahing; Yang, Hong; Gan, Jingsi; Li, Yanru
• Is Part Of: Food chemistry. Volume 225(2017)
• Page Start: 267
• Page End: 275
• Publication Date: 2017-06-15
• Subjects: d-glucose (PubChem CID: 5793) -- d-galactose (PubChem CID: 6036) -- d-mannose (PubChem CID: 18950) -- d-xylose (PubChem CID: 135191) -- d-arabinose (PubChem CID: 229) -- d-rhamnose (PubChem CID: 5460029) -- Trifluoroacetic acid (PubChem CID: 6422) -- Pyridine (PubChem CID: 1049) -- Hydroxylamine hydrochloride (PubChem CID: 443297) -- Sodium periodate (PubChem CID: 23667635) -- Acetic anhydride (PubChem CID: 7918) -- Ethylene glycol (PubChem CID: 174) -- Sodium hydroxide (PubChem CID: 14798) -- Methyl iodide (PubChem CID: 6328) -- Dimethyl sulfoxide (PubChem CID: 679) -- DMSO-d6 (PubChem CID: 75151); Lignosus rhinocerotis -- β-d-glucan -- Hyperbranched polysaccharide -- Compact coil -- Chain conformation -- Fractal dimension; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.01.034
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: LRP was extracted from the sclerotia of Lignosus rhinocerotis with 0.5 M NaOH. LRP was a (1 → 3)-β-glucan backbone bearing a hyperbranched side chain linked at O6. The hyperbranched side chain had two (1 → 3, 6)-β- and three (1 → )-β-glucose residues. Mw, < S 2 > z 1/2, Rh and [ η ] of LRP were 2.88 × 10 5 g/mol, 30.36 nm, 22.34 nm and 131.50 ml/g. Based on α, β, df and ρ, LRP (DB = 0.76) adopted compact coil conformation in LiCl/DMSO. Abstract: An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC–MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1 → 3)-β-d -Glcp backbone with every three residues bearing a (1 → 6)-linked and hyperbranched side chain that contained three (1 → 6)-β-d -Glcp residues as secondary main chain and two terminal β-d -Glcp residues linked at O3. The degree of branching was 0.76 from GC–MS analysis, implying a highly branched structure for LRP. The Mw, < S 2 > z 1/2, Rh and [ η ] values of LRP in 0.25 M LiCl/DMSO were measured by SEC-MALLS-Vis-RI combination technology to be 2.88 × 10 5 g/mol, 30.36 nm, 22.34 nm and 131.50 ml/g, respectively. Furthermore, the exponent α of [ η ] –Mw, β of < S 2 > z 1/2 –Mw, the fractal dimension df and molecular parameter ρ were determined to be 0.20, 0.33, 2.50 and 1.36, demonstrating that the LRP was a hyperbranched polysaccharide and adopted a compact coil conformation in LiCl/DMSO.