Untargeted metabolomics based on ultra-high-performance liquid chromatography–high-resolution mass spectrometry merged with chemometrics: A new predictable tool for an early detection of mycotoxins. (1st June 2017)

• Record Type: Journal Article
• Authors: Rubert, Josep; Righetti, Laura; Stranska-Zachariasova, Milena; Dzuman, Zbynek; Chrpova, Jana; Dall'Asta, Chiara; Hajslova, Jana
• Is Part Of: Food chemistry. Volume 224(2017)
• Page Start: 423
• Page End: 431
• Publication Date: 2017-06-01
• Subjects: Alkylresorcinols -- Cereals -- Lipidomics -- Liquid chromatography-high resolution mass spectrometry -- Metabolomics -- Oxylipins -- Plant-pathogen cross-talk -- Wheat; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.11.132
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Mycotoxins, Fusarium spp. and metabolic pathways were jointly investigated. SLE-UHPLC-HRMS untargeted metabolomics revealed cross-talk plant pathogen. Strong infection was associated with oxylipin and phosphatidic acid pathways. Low infection showed up higher levels of alkylresorcinols and phosphatidic acid. Abstract: In order to explore the early detection of mycotoxins in wheat three standardized approaches ( Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxin quantification) and a novel untargeted metabolomics strategy were jointly assessed. In the first phase of this research, standardized approaches were able to quantify mycotoxins and identify Fusarium spp. Then, an UHPLC-QTOF metabolic fingerprinting method was developed to investigate plant-pathogen cross-talk. At the same time, chemometrics analysis demonstrated to be a powerful tool in order to distinguish low and strong infection levels. Combining these results, the cross-talk plant pathogen related to the early detection of mycotoxins was discovered. As a rapid response to fungal infection an overexpression of phosphatidic acids was discovered. By contrast, when the infection became stronger an increase of oxylipins and diacylglycerols was revealed.