Piceatannol exhibits anti‐inflammatory effects on macrophages interacting with adipocytes. Issue 1 (16th May 2016)

• Record Type: Journal Article
• Authors: Yamamoto, Takayuki; Li, Yongjia; Hanafusa, Yuki; Yeh, Yu‐Sheng; Maruki‐Uchida, Hiroko; Kawakami, Shinpei; Sai, Masahiko; Goto, Tsuyoshi; Ito, Tatsuhiko; Kawada, Teruo
• Is Part Of: Food science & nutrition. Volume 5:Issue 1(2017)
• Page Start: 76
• Page End: 85
• Publication Date: 2016-05-16
• Subjects: 3T3‐L1 Adipocytes -- C3H10T1/2 adipocytes -- inflammation -- piceatannol -- RAW264.7 macrophages -- UCP1; Food industry and trade -- Periodicals; Food -- Periodicals; Nutrition -- Periodicals; 664
• DOI: 10.1002/fsn3.366
• Languages: English
• ISSNs: 2048-7177

Abstract

Abstract : Piceatannol exhibit beneficial anti‐inflammatory effects in a macrophage/adipocyte model of chronic inflammation, and improve the chronic inflammation‐induced adipocyte dysfunction such as suppression of uncoupling protein 1 induction. Abstract: Piceatannol (PIC), a natural analog of resveratrol (RES), is a phytochemical found in passion fruit seeds. To clarify the effects of PIC on obesity‐induced inflammation in adipose tissue, we investigated the anti‐inflammatory activity of PIC‐related compounds (PIC, RES, and metabolites from PIC) in culture models of obese adipose tissue. Lipopolysaccharide (LPS) and conditioned medium from 3T3‐L1 adipocytes (3T3‐L1‐CM) enhanced proinflammatory gene expression and synthesis of nitric oxide (NO), tumor necrosis factor‐ α (TNF‐ α ), and interleukin‐6 (IL‐6) in RAW264.7 macrophages. Although each compound inhibited the mRNA expression of iNOS (inducible NO synthase), TNF‐ α, and IL‐6, PIC potently inhibited them, and 30 μ mol/L PIC suppressed the LPS‐ and 3T3‐L1‐CM‐induced mRNA expression of iNOS (70.4% and 69.2% suppression, respectively), TNF‐ α (42.6% and 47.0% suppression), and IL‐6 (27.3% and 42.1% suppression). PIC also significantly suppressed production of NO (80.3% suppression) and inflammatory cytokines (TNF‐ α ; 33.7% suppression, IL‐6; 66.5% suppression). Furthermore, PIC was found to rescue the uncoupling protein 1 mRNA expression induced by isoproterenol in 10T1/2 adipocytes, which was suppressed by LPS‐activated macrophages. These results suggest that PIC may attenuate the pathologic inflammation triggered by adipose tissues.