Production of d-ribose by metabolically engineered Escherichia coli. (January 2017)

• Record Type: Journal Article
• Authors: Park, Hae-Chul; Kim, Yun-Jung; Lee, Chang-Wan; Rho, Yong-Taek; Kang, JeongWoo; Lee, Dae-Hee; Seong, Yeong-Je; Park, Yong-Cheol; Lee, Daesang; Kim, Sung-Gun
• Is Part Of: Process biochemistry. Volume 52(2017)
• Page Start: 73
• Page End: 77
• Publication Date: 2017-01
• Subjects: d-Ribose -- Xylose -- Escherichia coli -- Transketolase -- Catabolite repression; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.10.001
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Transketolase-deficient E. coli was constructed to produced -ribose from xylose. d -Ribose produced in the E. coli was identical to standardd -ribose in HPLC and LC/MS analyses. The transketolase-deficient E. coli was further engineered to uptake glucose and xylose simultaneously for facilitatingd -ribose production. The simultaneous uptake of glucose and xylose in the E. coli resulted in a 5-fold improvement ofd -ribose production. Abstract: Escherichia coli was metabolically engineered for the production ofd -ribose, a functional five-carbon sugar, from xylose. For the accumulation ofd -ribose, two genes of transketolase catalyzing the conversion ofd -ribose-5-phosphate to sedoheptulose-7-phosphate in pentose phosphate pathway were disrupted to create a transketolase-deficient E. coli SGK013. In batch fermentation, E. coli SGK013 grew by utilizing glucose and then started to produced -ribose from xylose after glucose depletion. E. coli SGK013 produced 0.75 g/L ofd -ribose, which was identical to the standardd -ribose as confirmed by HPLC and LC/MS analyses. To improve D-ribose production, the ptsG gene encoding the glucose-specific IICB component was disrupted additionally, resulting in the construction of E. coli SGK015. The carbon catabolite repression-negative E. coli SGK015 utilized xylose and glucose simultaneously and produced up to 3.75 g/L ofd -ribose, which is a 5-fold improvement compared to that of E. coli SGK013.