Application of TMA-PEG to promote C-phycocyanin extraction from S. platensis in the PEG ATPS. (January 2017)

• Record Type: Journal Article
• Authors: Wang, Feng; Liu, Yan-Hua; Ma, Yong; Cui, Zheng-Gang; Shao, Lin-Lin
• Is Part Of: Process biochemistry. Volume 52(2017)
• Page Start: 283
• Page End: 294
• Publication Date: 2017-01
• Subjects: ATPS aqueous two-phase -- C-PC C-phycocyanin -- TMA-PEG trimethylamine-polyethylene glycol -- PEG polyethylene glycol -- RSM Response Surface Method -- CCD counter-current distribution; C-phycocyanin -- ATPS -- TMA-PEG -- Purity -- Recovery -- Extraction; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2016.11.006
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: The purity of C-PC in ATPS was increased by 2.1 times in the presence of TMA-PEG. The promoting effect of TMA-PEG addition was based on the electrostatic attraction. The purity of C-PC was increased by 18.9% using RSM optimization. C-PC with a purity of 6.7 can be obtained in the CCD operation of 2-stage ATPS. Abstract: A novel aqueous two phase system (ATPS) using trimethylamine-polyethylene glycols (TMA-PEG) to promote the extraction of C-phycocyanin (C-PC) from S.platensis was introduced. The purity of C-PC ( EP ) obtained in the ATPS of PEG1000/Na3 PO4 was increased 2.1 times by the addition of TMA-PEG1000. The purification factor was enhanced from 2.9 to 10.1 when 65% TMA-PEG1000 was added in the system. The ATPS operation must be carried out in the pH range of 6.0-7.0 and at temperatures less than 35 °C for maintaining the stability of C-PC. The partition coefficient and recovery ratio of C-PC increased with the increasing concentration of TMA-PEG. The system parameters like TMA-PEG1000 content, tie line length ( TLL ), pH, temperature and phase volume ratio ( Vr ) were screened and optimized using the fractional factorial design and Box-Behnken experiment design. The optimized system is composed of 11.8% PEG1000/TMA-PEG1000 (w/w), 64.42% TMA-PEG1000 (w/w PEG1000) and 9.5% Na3 PO4 (w/w) with 38.19% TLL (w/w) and 0.89 Vr at pH 6.5 and 25 °C. The obtained value of EP was 5.21 in one-stage ATPS and 6.7 in two-stage ATPS. The recovery ratio of C-PC in the new ATPS extraction system was more than 97%.