Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples. (1st May 2017)

• Record Type: Journal Article
• Authors: Ng, Nyuk Ting; Sanagi, Mohd Marsin; Wan Ibrahim, Wan Nazihah; Wan Ibrahim, Wan Aini
• Is Part Of: Food chemistry. Volume 222(2017)
• Page Start: 28
• Page End: 34
• Publication Date: 2017-05-01
• Subjects: Phenanthrene (PubChem CID: 995) -- Pyrene (PubChem CID: 31423); Agarose-chitosan-immobilized C18 adsorbent -- Micro-solid phase extraction -- Chrysanthemum tea -- Phenanthrene -- Pyrene; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.11.147
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Agarose-chitosan-C18 film micro-solid phase extraction was developed. Agr-Ch-C18 -μSPE employed low-cost and eco-friendly agarose and chitosan as sorbent. It was used for the determination of phenanthrene and pyrene in chrysanthemum tea. Results showed that Agr-Ch-C18 -μSPE achieved high analyte recoveries. It proved a good sample preparation for the analysis of PAH in tea samples. Abstract: Agarose-chitosan-immobilized octadecylsilyl-silica (C18 ) film micro-solid phase extraction (μSPE) was developed and applied for the determination of phenanthrene (PHE) and pyrene (PYR) in chrysanthemum tea samples using high performance liquid chromatography-ultraviolet detection (HPLC-UV). The film of blended agarose and chitosan allows good dispersion of C18, prevents the leaching of C18 during application and enhances the film mechanical stability. Important μSPE parameters were optimized including amount of sorbent loading, extraction time, desorption solvent and desorption time. The matrix match calibration curves showed good linearity ( r ⩾ 0.994) over a concentration range of 1–500 ppb. Under the optimized conditions, the proposed method showed good limits of detection (0.549–0.673 ppb), good analyte recoveries (100.8–105.99%) and good reproducibilities (RSDs ⩽ 13.53%, n = 3) with preconcentration factors of 4 and 72 for PHE and PYR, respectively.