His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells. Issue 44 (2nd November 2016)

• Record Type: Journal Article
• Authors: Zheng, Lin Ling; Li, Chun Mei; Zhen, Shu Jun; Li, Yuan Fang; Huang, Cheng Zhi
• Is Part Of: Nanoscale. Volume 8:Issue 44(2016)
• Page Start: 18635
• Page End: 18639
• Publication Date: 2016-11-02
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c6nr05806j
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Progeny viruses were labelled with His-tags by in situ modifying cell surface proteins during progeny virus assembly, and conjugated with QD–NTA–Ni 2+ for further real-time single virus tracking in living cells. Abstract : Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni 2+ –nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.