His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells. Issue 44 (2nd November 2016) - Zheng, Lin Ling
Abstract : Progeny viruses were labelled with His-tags by in situ modifying cell surface proteins during progeny virus assembly, and conjugated with QD–NTA–Ni 2+ for further real-time single virus tracking in living cells. Abstract : Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni 2+ –nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.