Conjugated linoleic acid rat pretreatment reduces renal damage in ischemia/reperfusion injury: Unraveling antiapoptotic mechanisms and regulation of phosphorylated mammalian target of rapamycin. Issue 12 (30th September 2016)

• Record Type: Journal Article
• Authors: Javedan, Gholamali; Shidfar, Farzad; Davoodi, Sayed Hossein; Ajami, Marjan; Gorjipour, Fazel; Sureda, Antoni; Nabavi, Seyed Mohammad; Daglia, Maria; Pazoki‐Toroudi, Hamidreza
• Is Part Of: Molecular nutrition & food research. Volume 60:Issue 12(2016)
• Page Start: 2665
• Page End: 2677
• Publication Date: 2016-09-30
• Subjects: Apoptosis -- Conjugated linoleic acids -- Oxidative stress -- Renal ischemia -- Renal function -- Reperfusion injury; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201600112
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : This study aims to evaluate the potential benefits of conjugated linoleic acids pretreatment in a rat model of renal ischemia/reperfusion injury (IRI). The results suggest that in rats pretreated with conjugated linoleic acids (CLAs) before renal IRI induction, decreased renal tissue damage, improved renal function and oxidative stress are registered. These results are associated with downregulation of Bax and P‐mTOR, and upregulation of Bcl‐2. CLA pretreatment results in protection against IRI through the regulation of signaling pathways involved in apoptosis. Abstract : Scope: Conjugated linoleic acids (CLAs) are dietary components with beneficial effects on human health. The aim of this study was to evaluate the potential benefits of CLA pretreatment in a rat model of renal ischemia/reperfusion injury (IRI). Methods and results: Animals were treated with CLAs (200 mg/kg/day) or water for two weeks prior to sham surgery or to surgery to induce IRI. Renal function, oxidative stress, apoptosis, and cell proliferation markers, were evaluated. Moreover, kidney sections were submitted to histological evaluation. IRI induced increased serum creatinine, blood urea nitrogen, fractional sodium excretion, malondialdehyde, Bax, and phosphorylated mammalian target of rapamycin (P‐mTOR), and decreased clearance of creatine, superoxide dismutase and catalase activities, and Bax in comparison with control groups. CLA prefeeding restored, at least in part, the above reported markers to normal levels, increased the anti‐apoptotic protein, B‐cell lymphoma 2 (Bcl‐2), and reduce the histological damage. Conclusion: The results suggest that the decreased renal tissue damage and improved renal function and oxidative stress, in rats pretreated with CLAs before renal IRI induction, could be associated with downregulation of Bax and P‐mTOR, and upregulation of Bcl‐2. CLAs pretreatment resulted to protect against IRI through the regulation of signaling pathways involved in apoptosis.