The potential beneficial effects of phenolic compounds isolated from A. pilosa Ledeb on insulin-resistant hepatic HepG2 cells. Issue 10 (21st September 2016)

• Record Type: Journal Article
• Authors: Teng, Hui; Chen, Lei; Song, Hongbo
• Is Part Of: Food & function. Volume 7:Issue 10(2016)
• Page Start: 4400
• Page End: 4409
• Publication Date: 2016-09-21
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c5fo01067e
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Agrimonia pilosa Ledeb (AP) has already been applied in practice for the treatment of different disorders and is available to access without the provision of a medical prescription. Abstract : Agrimonia pilosa Ledeb (AP) has already been applied in practice for the treatment of different disorders and is available to access without the provision of a medical prescription. The present study aims at investigating the effect of bioactive compounds isolated from AP on the improvement of insulin resistance, figuring out the mechanism in insulin-responsive cell lines. Five compounds were isolated from AP using column chromatography, including agrimonolide (K1 ), desmethylagrimonolide (K2 ), tormentic acid (K3 ), ursolic acid (K4 ), and quercetin (K5 ). Glucose metabolism was evaluated in insulin-resistant HepG2 cells. Ursolic acid had the strongest activity among all isolated compounds with the lowering value of 71.5% (1.24 mM glucose in DMEM) and 71.7% (1.23 mM) when compared to the control.K1 consisting ofK2 effectively increased the insulin-mediated glycogen level in hepatocytes. At a concentration level of 20 μM, K2 significantly elevated the hepatic glucokinase (GK) activity (3.2 U min −1 mg −1 protein), followed byK1 (3.0 U min −1 mg −1 protein). Both of them significantly increased ( p < 0.05) the GK activity as compared to the control. On the same lines, K2 andK1 caused a significant reduction of the glucose-6-phosphatase (G6Pase) activity and a significant change in the phosphoenolpyruvate carboxykinase (PEPCK) activity. In summary, bioactive compounds in AP may play an important role in regulating the glucose metabolism in insulin-resistant HepG2 cells and could be developed as a promising natural material for diabetes prevention and treatment.