Microbial hydroxylation of 17β-estradiol by Penicillium brevicompactum. Issue 4 (3rd July 2016)

• Record Type: Journal Article
• Authors: Shan, Lihong; Li, Yang; Chen, Yanjie; Yin, Minghui; Huang, Jiajia; Zhang, Zhenzhong; Shi, Xiufang; Liu, Hongmin
• Is Part Of: Biocatalysis and biotransformation. Volume 34:Issue 4(2016)
• Page Start: 137
• Page End: 143
• Publication Date: 2016-07-03
• Subjects: Biotransformation -- 17β-estradiol -- hydroxylation -- Penicillium brevicompactum; Enzymes -- Biotechnology -- Periodicals; Enzymes -- Industrial applications -- Periodicals; Biotransformation (Metabolism) -- Periodicals; 660.63
• DOI: 10.1080/10242422.2016.1247816
• Languages: English
• ISSNs: 1024-2422

Abstract

Abstract: Microbial hydroxylation of 17β-estradiol (1 ) with Penicillium brevicompactum, a fungal species not used in biotransformation so far, yielded four metabolites: 1, 3, 5-estratriene-3, 15α-diol-17-one (2 ); 1, 3, 5-estratriene-3, 6α, 17β-triol (3 ); 1, 3, 5-estratriene-3, 15α, 17β-triol (4 ); and 1, 3, 5-estratriene-3, 6α, 15α-triol-17-one (5 ). All the products were determined by 1 H NMR, 13 C NMR, two-dimensional NMR, and HRMS techniques. Compounds3, 4, and5 are reported for the first time via microbial transformation, and5 is a new compound as far as we know. Possible metabolic pathway of 17β-estradiol via Penicillium brevicompactum was also proposed.