Biotransformation of 5-en-3β-ol steroids by Mucor circinelloides lusitanicus. Issue 3 (3rd May 2016)

• Record Type: Journal Article
• Authors: Shan, Lihong; Jiao, Kai; Yin, Minghui; Huang, Jiajia; Chen, Yanjie; Qin, Shangshang; Liu, Hongmin
• Is Part Of: Biocatalysis and biotransformation. Volume 34:Issue 3(2016)
• Page Start: 83
• Page End: 88
• Publication Date: 2016-05-03
• Subjects: biotransformation -- 5-en-3β-ol steroids -- 7α-hydroxylation -- 11α-hydroxylation -- Mucor circinelloides lusitanicus; Enzymes -- Biotechnology -- Periodicals; Enzymes -- Industrial applications -- Periodicals; Biotransformation (Metabolism) -- Periodicals; 660.63
• DOI: 10.3109/10242422.2015.1089865
• Languages: English
• ISSNs: 1024-2422

Abstract

Abstract: In this work, we report the mode of biotransformation of 5-en-3β-ol steroids using Mucor circinelloides lusitanicus for the first time. Here, we selected seven 5-en-3β-ol steroids as substrates. The main characteristic of the fungus was to introduce a 7α-hydroxyl group into substrates1 --5 . With substrate2, 3β, 7α, 11α-trihydroxypregna-5-en-20-one (2b ) was obtained as the final product in good yield (46.4%). All the metabolites were determined by infrared spectra, high-resolution mass spectrometry, proton nuclear magnetic resonance, and carbon-13 nuclear magnetic resonance.