Biodegradation of 1, 1, 1-trichloro-2, 2-bis(4-chlorophenyl) ethane (DDT) by using Serratia marcescens NCIM 2919. Issue 12 (1st December 2016)

• Record Type: Journal Article
• Authors: , Neerja; Grewal, Jasneet; Bhattacharya, Amrik; Kumar, Sumit; Singh, Dileep K.; Khare, Sunil K.
• Is Part Of: Journal of environmental science and health. Volume 51:Issue 12(2016)
• Page Start: 809
• Page End: 816
• Publication Date: 2016-12-01
• Subjects: DDT -- biodegradation -- Serratia marcescens -- pathway -- metabolites; Pesticides -- Environmental aspects -- Periodicals; Food contamination -- Periodicals; Agricultural wastes -- Environmental aspects -- Periodicals; Ecology -- periodicals; Pesticides -- toxicity -- periodicals; Food Contamination -- periodicals; 363.73
• DOI: 10.1080/03601234.2016.1208455
• Languages: English
• ISSNs: 0360-1234

Abstract

ABSTRACT: A solvent tolerant bacterium Serratia marcescens NCIM 2919 has been evaluated for degradation of DDT (1, 1, 1-trichloro-2, 2- bis (4-chlorophenyl) ethane). The bacterium was able to degrade up to 42% of initial 50 mg L −1 of DDT within 10 days of incubation. The highlight of the work was the elucidation of DDT degradation pathway in S. marcescens. A total of four intermediates metabolites viz. 2, 2- bis (chlorophenyl)-1, 1-dichloroethane (DDD), 2, 2- bis (chlorophenyl)-1, 1-dichloroethylene (DDE), 2, 2- bis (chlorophenyl)-1-chloroethylene (DDMU), and 4-chlorobenzoic acid (4-CBA) were identified by GC-Mass and FTIR. 4-CBA was found to be the stable product of DDT degradation. Metabolites preceding 4-CBA were not toxic to strain as reveled through luxuriant growth in presence of varying concentrations of exogenous DDD and DDE. However, 4-CBA was observed to inhibit the growth of bacterium. The DDT degrading efficiency of S. marcescens NCIM 2919 hence could be used in combination with 4-CBA utilizing strains either as binary culture or consortia for mineralization of DDT. Application of S. marcescens NCIM 2919 to DDT contaminated soil, showed 74.7% reduction of initial 12.0 mg kg −1 of DDT after 18-days of treatment.