Biodegradation of 1, 1, 1-trichloro-2, 2-bis(4-chlorophenyl) ethane (DDT) by using Serratia marcescens NCIM 2919. Issue 12 (1st December 2016) - , Neerja
ABSTRACT: A solvent tolerant bacterium Serratia marcescens NCIM 2919 has been evaluated for degradation of DDT (1, 1, 1-trichloro-2, 2- bis (4-chlorophenyl) ethane). The bacterium was able to degrade up to 42% of initial 50 mg L −1 of DDT within 10 days of incubation. The highlight of the work was the elucidation of DDT degradation pathway in S. marcescens. A total of four intermediates metabolites viz. 2, 2- bis (chlorophenyl)-1, 1-dichloroethane (DDD), 2, 2- bis (chlorophenyl)-1, 1-dichloroethylene (DDE), 2, 2- bis (chlorophenyl)-1-chloroethylene (DDMU), and 4-chlorobenzoic acid (4-CBA) were identified by GC-Mass and FTIR. 4-CBA was found to be the stable product of DDT degradation. Metabolites preceding 4-CBA were not toxic to strain as reveled through luxuriant growth in presence of varying concentrations of exogenous DDD and DDE. However, 4-CBA was observed to inhibit the growth of bacterium. The DDT degrading efficiency of S. marcescens NCIM 2919 hence could be used in combination with 4-CBA utilizing strains either as binary culture or consortia for mineralization of DDT. Application of S. marcescens NCIM 2919 to DDT contaminated soil, showed 74.7% reduction of initial 12.0 mg kg −1 of DDT after 18-days of treatment.