A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal. (15th January 2017)

• Record Type: Journal Article
• Authors: Chen, Lu; Wen, Fang; Li, Ming; Guo, Xiaodong; Li, Songli; Zheng, Nan; Wang, Jiaqi
• Is Part Of: Food chemistry. Volume 215(2017)
• Page Start: 377
• Page End: 382
• Publication Date: 2017-01-15
• Subjects: Aflatoxin B1 -- Aptamer -- Fluorescent -- Quenching -- Infant rice cereal; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.07.148
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A fluorescent aptasensor has been developed for detection of AFB1 in infant food. TAMRA at the end of probe plays as a quencher in fluorescent aptasensor. The signal increases with increasing the concentration of the AFB1. The sensor provides rapid, sensitive, and detection of AFB1 . This method has the potential for AFB1 analysis in diverse foods. Abstract: A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1 ) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5–100 ng/mL with a detection limit down to 1.6 ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%–106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.