Effects of pectin supplementation on the fermentation patterns of different structural carbohydrates in rats. Issue 10 (8th June 2016)

• Record Type: Journal Article
• Authors: Tian, Lingmin; Scholte, Jan; Borewicz, Klaudyna; van den Bogert, Bartholomeus; Smidt, Hauke; Scheurink, Anton J.W.; Gruppen, Harry; Schols, Henk A.
• Is Part Of: Molecular nutrition & food research. Volume 60:Issue 10(2016)
• Page Start: 2256
• Page End: 2266
• Publication Date: 2016-06-08
• Subjects: Digestibility -- Gut microbiota -- MiSeq sequencing -- Pectin -- SCFA; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201600149
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : The effects of four types of pectins on dietary fiber (DF) fermentation, microbiota composition, and short chain fatty acid (SCFA) production throughout the large intestine in male Wistar rats are investigated and compared. The fermentation patterns of cereal arabinoxylans in the cecum are significantly different upon supplementation of different pectins. These differences, however, become smaller in the colon due to an enhanced fermentation of the remaining DFs. The results show that dietary supplementation of pectin is a potential strategy to modulate the location of fermentation of DFs, and consequently microbiota composition and SCFA production for health‐promoting effects. Abstract : Scope: We aimed to investigate and compare the effects of four types of pectins on dietary fiber (DF) fermentation, microbiota composition, and short chain fatty acid (SCFA) production throughout the large intestine in rats. Methods and results: Male Wistar rats were given diets supplemented with or without 3% structurally different pectins for 7 weeks. Different fermentation patterns of pectins and different location of fermentation of pectin and diet arabinoxylans (AXs) in the large intestine were observed. During cecal fermentation, sugar beet pectin significantly stimulated Lactobacillus ( p < 0.01) and Lachnospiraceae ( p < 0.05). The stimulating effects of sugar beet pectin on these two groups of microbes are stronger than both other pectins. In the cecum, low‐methyl esterified citrus pectin and complex soy pectin increased ( p < 0.05) the production of total SCFAs, propionate and butyrate, whereas high‐methyl esterified pectin and sugar beet pectin did not. The fermentation patterns of cereal AXs in the cecum were significantly different upon supplementation of different pectins. These differences, however, became smaller in the colon due to an enhanced fermentation of the remaining DFs. Conclusion: Dietary supplementation of pectin is a potential strategy to modulate the location of fermentation of DFs, and consequently microbiota composition and SCFA production for health‐promoting effects.