A fast and environment-friendly MEPSPEP/UHPLC-PDA methodology to assess 3-hydroxy-4, 5-dimethyl-2(5H)-furanone in fortified wines. (1st January 2017)

• Record Type: Journal Article
• Authors: Freitas, Jorge; Perestrelo, Rosa; Cassaca, Rubina; Castillo, Mariangie; Santos, Mariana; Pereira, Jorge; Câmara, José S.
• Is Part Of: Food chemistry. Volume 214(2017)
• Page Start: 686
• Page End: 693
• Publication Date: 2017-01-01
• Subjects: ACN acetonitrile -- BIN barrel insert and needle -- D/MD dry/medium dry Madeira wines -- DCM dicloromethane -- FA formic acid -- GC-MS gas chromatography – mass spectrometry -- LC-PDA liquid chromatography with photodiode detection -- LLE liquid-liquid extraction -- LOD limit of detection -- LOQ limit of quantification -- MeOH methanol -- MEPSPEP microextraction by packed sorbent using the PEP sorbent -- PS/DVB porous polystyrene–divinylbenzene polymer -- premox premature oxidative wine aging -- S/MS sweet/medium sweet Madeira wines -- SPE solid-phase extraction -- UHPLC-MS ultra-high pressure liquid chromatography-mass spectrometry; Sotolon -- Wine -- Oxidative aging -- Premox -- Microextraction by packed sorbent (MEPS); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.07.107
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An innovative strategy, MEPSPEP /UHPLC, for sotolon identification in wines. Attractive, promising and reliable alternative to commonly used methods. Unparalleled wine sample and organic solvents usage, reducing the analysis cost. Good results for selectivity, linearity, precision, accuracy and matrix effect. A rapid screening for quantification of sotolon in fortified and table wines. Abstract: Sotolon is widely associated with the quality of fortified aged wines, and has also been linked to premature oxidative aging ( premox ). Here we developed a single, fast and environmental-friendly microextraction by packed sorbent ultra-high pressure liquid chromatography analysis (MEPS/UHPLC-PDA) for sotolon quantification in different wines. The best extraction conditions (loading three times 250 μL samples through the MEPSPEP sorbent and elution with 100 μL of 50% MeOH) were combined with a fast UHPLC separation (5 min separation using acidified 10% MeOH isocratic flow in a CORTECS C18 column) to allow unparalleled minimum sample and solvents volumes usage. The validated methodology showed good linearity ( r 2 > 0.993) and precision (<5.6%); high recovery (>81%) and detection limits (0.45–2.51 μg/L) far below sotolon odor threshold for any type of wine. The methodology was successfully applied to selected white table and Madeira wines, encompassing therefore a wide range of alcohol and sugar contents. Furthermore, as far we may know, this is the first time a single methodology can be used to assess both wine aging or premox according to the type of wine.