Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples. (15th February 2017)

• Record Type: Journal Article
• Authors: Guo, Pengqi; Luo, Zhimin; Xu, Xinya; Zhou, Yulan; Zhang, Bilin; Chang, Ruimiao; Du, Wei; Chang, Chun; Fu, Qiang
• Is Part Of: Food chemistry. Volume 217(2017)
• Page Start: 628
• Page End: 636
• Publication Date: 2017-02-15
• Subjects: Clenbuterol -- Molecular imprinted monolithic columns -- Two-dimensional liquid chromatography -- Pork liver -- Swine urine; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.09.021
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A novel MIPs-2D-LC analytical system was developed and validated for the first time. The frontal analysis was used to characterize the imprinted columns. The developed method was used to analyze the clenbuterol in liver and urine samples. Abstract: A novel method coupling molecular imprinted monolithic column with two-dimensional liquid chromatography was developed and validated for the analysis of clenbuterol in pork liver and swine urine samples. The polymers were characterized by using Fourier transform infrared spectroscopy, nitrogen adsorption desorption analyses, frontal analysis and the adsorption of selectivity. The results indicated that the imprinted columns were well prepared and possessed high selectivity adsorption capacity. Subsequently, the MIMC-2D-LC (molecular imprinted monolithic column-two dimensional liquid chromatography) method was developed for the selective analysis of clenbuterol in practical samples. The accuracy ranged from 94.3% to 99.7% and from 93.7% to 99.6% for liver and urine, respectively. The relative standard deviation (RSD) of repeatability was lower than 8.6% for both analyses. The limit of detections was 16 ng·mL −1 for liver and 25 ng·mL −1 for urine, respectively. Compared with the reported methods, the disturbance of endogenous impurity could be avoided by the 2D-LC method.