Citrus monstruosa Discrimination among Several Citrus Species by Multivariate Analysis of Volatiles: A Metabolomic Approach. Issue 5 (3rd December 2015)

• Record Type: Journal Article
• Authors: Petretto, Giacomo Luigi; Sarais, Giorgia; Maldini, Maria Teresa; Foddai, Marzia; Tirillini, Bruno; Rourke, Jonathan P.; Chessa, Mario; Pintore, Giorgio
• Is Part Of: Journal of food processing and preservation. Volume 40:Issue 5(2016:Oct.)
• Page Start: 950
• Page End: 957
• Publication Date: 2015-12-03
• Subjects: Food -- Preservation -- Periodicals; Food industry and trade -- Periodicals; 664.005
• DOI: 10.1111/jfpp.12674
• Languages: English
• ISSNs: 0145-8892

Abstract

Abstract: The origin and taxonomy of C itrus monstruosa (Rutaceae) are currently unknown and thus a nontargeted metabolomic analysis on the flavedo volatiles was performed. Headspace solid‐phase micro extraction coupled with gas chromatography analysis was applied to eight C itrus spp. including Citrus monstruosa, and the chemical composition of the volatile organic compounds (VOC) was analyzed to give a chemical comparison between different C itrus species. Forty‐four metabolites were detected across the species: several monoterpenes were found in all C itrus spp. and among them limonene comes first in all C itrus spp. ranging from 96.7 to 66.7%. Conversely, some molecules were found to be specific for only a few of the samples. The classical univariate evaluation of the VOC data is unlikely to give objective data for the cultivar classification; a multivariate approach, principal component analysis, is an easy to use, powerful tool. Therefore, a chemometric comparison between chromatographic data of all C itrus spp. was performed with the aim of understanding the role of VOC in the discrimination of the eight studied cultivars. Practical Application: A chemometrics' method was applied on the volatiles of eight C itrus spp. including C itrus monstruosa . Results were used to discriminate and classify C . monstruosa from several other C itrus spp. This method may also be used to discriminate other C itrus spp. as well as being applied to the volatiles‐based classification of other botanicals.