Potential transfer of extended spectrum β-lactamase encoding gene, blashv18 gene, between Klebsiella pneumoniae in raw foods. (December 2016)

• Record Type: Journal Article
• Authors: Jung, Yangjin; Matthews, Karl R.
• Is Part Of: Food microbiology. Volume 60(2016:Dec.)
• Page Start: 39
• Page End: 48
• Publication Date: 2016-12
• Subjects: Extended-spectrum β-lactamase(s) (ESBLs) -- Klebsiella pneumoniae -- Antibiotic resistant gene transfer -- Transfer frequency -- Seed sprouts -- Lettuce; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2016.06.002
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: This study investigated the transfer frequency of the extended-spectrum β-lactamase-encoding gene ( bla SHV18 ) among Klebsiella pneumoniae in tryptic soy broth (TSB), pasteurized milk, unpasteurized milk, alfalfa sprouts and chopped lettuce at defined temperatures. All transconjugants were characterized phenotypically and genotypically. KP04 ΔKM and KP08 ΔKM isolated from seed sprouts and KP342 were used as recipients in mating experiments with K. pneumoniae ATCC 700603 serving as the donor. In mating experiments, no transconjugants were detected at 4 °C in liquid media or chopped lettuce, but detected in all media tested at 15 °C, 24 °C, and 37 °C. At 24 °C, the transfer of bla SHV18 gene occurred more frequently in alfalfa sprouts (5.15E-04 transconjugants per recipient) and chopped lettuce (3.85E-05) than liquid media (1.08E-05). On chopped lettuce, transconjugants were not detected at day 1 post-mating at 15 °C, but observed on day 2 (1.43E-05). Transconjugants carried the bla SHV18 gene transferred from the donor and the virulence gene harbored by recipient. More importantly, a class 1 integrase gene and resistance to tetracycline, trimethoprim/sulfamethoxazole were co-transferred during mating. These quantitative results suggest that fresh produce exposed to temperature abuse may serve as a competent vehicle for the spread of gene encoding for antibiotic resistance, having a potential negative impact on human health. Highlights: Seed sprouts contain abundant ceftazidime resistant Enterobacteriaceae. No transfer of the bla shv18 gene was observed at 4 °C. Commodities including alfalfa sprouts and shredded lettuce served as an efficient media of antibiotic resistance gene transfer. Prediction of transfer frequency of antibiotic resistance gene(s) may be underestimated in broth nutrient media.