Inhibition of PDGF‐induced migration and TNF‐α‐induced ICAM‐1 expression by maltotetraose from bamboo stem extract (BSE) in mouse vascular smooth muscle cells. Issue 9 (25th May 2016)

• Record Type: Journal Article
• Authors: Shin, Soon Young; Jung, You Jung; Yong, Yeonjoong; Cho, Hi Jae; Lim, Yoongho; Lee, Young Han
• Is Part Of: Molecular nutrition & food research. Volume 60:Issue 9(2016)
• Page Start: 2086
• Page End: 2097
• Publication Date: 2016-05-25
• Subjects: Atherosclerosis -- Bamboo stem extract -- intercellular adhesion molecule‐1 -- Platelet‐derived growth factor -- Vascular smooth muscle cells; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201500601
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Bambusae caulis in liquamen (BCL) is a nutritious liquid extracted from heat‐treated stems of bamboo species. The effect of BCL on the migration of VSMCs has not been well characterized. This study shows that BSE inhibited PDGF‐induced migration of vascular smooth muscle cells (VSMC), suppress TNF‐α‐induced ICAM‐1 expression by inhibiting NF‐κB activity in VSMC, and reduce adhesion between VSMC and monocytes. It is found that maltotetraose is a major functional constituent of BSE responsible for inhibition of migration and ICAM‐1 expression in VSMC. Abstract : Scope: Expression of intercellular adhesion molecule‐1 (ICAM‐1) on vascular smooth muscle cells (VSMCs) plays an important role in the progression of atherosclerosis. We investigated the effects of bamboo stem extract (BSE) on motility and ICAM‐1 expression by using mouse MOVAS‐1 cells. Active constituents of BSE exhibiting an inhibitory activity on TNF‐α‐induced ICAM1 expression were identified using HPLC. Methods and results: The effects of BSE on platelet‐derived growth factor (PDGF)‐BB‐induced migration, tumor necrosis factor alpha (TNF‐α)‐induced expression of ICAM‐1, and nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) activation were investigated. BSE inhibited migration of MOVAS‐1 cells and sprout formation by mouse aorta explants. Reverse transcription PCR analysis and promoter reporter assays revealed that BSE suppressed ICAM‐1 expression by inhibiting NF‐κB activity. In addition, BSE reduced adhesion between VSMCs and monocytes. Several oligosaccharides were identified in BSE. Among the oligosaccharides contained in BSE, maltotetraose and stachyose were potent inhibitors of TNF‐α‐induced ICAM‐1 expression. We confirmed that maltotetraose reduced PDGF‐induced sprout formation by mouse aorta explants and inhibited TNF‐α‐induced NF‐κB activation and ICAM‐1 expression in MOVAS‐1 cells. Conclusion: The BSE constituent maltotetraose may be beneficial in the suppression of early atherosclerosis development and could be developed as a dietary supplement for cardiovascular health.