Highly solvatochromic and tunable fluorophores based on a 4, 5-quinolimide scaffold: novel CDK5 probes12. Issue 62 (7th July 2016)

• Record Type: Journal Article
• Authors: González-Vera, Juan A.; Fueyo-González, Francisco; Alkorta, Ibon; Peyressatre, Marion; Morris, May C.; Herranz, Rosario
• Is Part Of: Chemical communications. Volume 52:Issue 62(2016)
• Page Start: 9652
• Page End: 9655
• Publication Date: 2016-07-07
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc04566a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Novel 4, 5-quinolimide-based solvatochromic fluorophores conjugated to a CDK5 derived peptide demonstrated sensitivity for monitoring CDK5/p25 interaction in living cells. Abstract : Novel 4, 5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introduction of the quinolimide-labelled peptide into living glioblastoma cells probed the interaction with endogenous p25.