Electrochemical Determination of 16s Ribosomal RNA of Mycobacterium Tuberculosis Using Magnetite on Silica with DNA-Functionalized Gold Nanoparticles. (12th June 2016)

• Record Type: Journal Article
• Authors: Sheng, Shangchun; Liu, Lingzhi; Zhao, Zhaohui; Cai, Miao; Jiang, Xiong; Kang, Yi; Dai, Qiong; Lu, Xinmin; Xie, Guoming
• Is Part Of: Analytical letters. Volume 49:Number 9(2016)
• Page Start: 1379
• Page End: 1387
• Publication Date: 2016-06-12
• Subjects: 16s Ribosomal RNA -- electrochemical biosensor -- gold nanoparticles -- magnetite -- mycobacterium tuberculosis; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2015.1101601
• Languages: English
• ISSNs: 0003-2719

Abstract

ABSTRACT: A new electrochemical biosensor for the determination of mycobacterium tuberculosis in clinical samples is reported. A sandwich hybridization layer system with magnetite on a silica nanocomposite was employed as the biosensor. The use of DNA-functionalized gold nanoparticles enhanced the signal of target 16 s ribosomal RNA of mycobacterium tuberculosis in clinical samples. When compared with the commonly used real-time fluorescent polymerase chain reaction, the analysis time was significantly reduced. A linear relationship was obtained for 16 s ribosomal RNA concentrations from 0 to 130 µM. This electrochemical biosensor provides rapid and simple determination of mycobacterium tuberculosis in clinical samples with high sensitivity and low cost.