Electrochemical Determination of 16s Ribosomal RNA of Mycobacterium Tuberculosis Using Magnetite on Silica with DNA-Functionalized Gold Nanoparticles. (12th June 2016) - Sheng, Shangchun
ABSTRACT: A new electrochemical biosensor for the determination of mycobacterium tuberculosis in clinical samples is reported. A sandwich hybridization layer system with magnetite on a silica nanocomposite was employed as the biosensor. The use of DNA-functionalized gold nanoparticles enhanced the signal of target 16 s ribosomal RNA of mycobacterium tuberculosis in clinical samples. When compared with the commonly used real-time fluorescent polymerase chain reaction, the analysis time was significantly reduced. A linear relationship was obtained for 16 s ribosomal RNA concentrations from 0 to 130 µM. This electrochemical biosensor provides rapid and simple determination of mycobacterium tuberculosis in clinical samples with high sensitivity and low cost.