Quantitative profiling of retinyl esters in milk from different ruminant species by using high performance liquid chromatography-diode array detection-tandem mass spectrometry. (15th November 2016)

• Record Type: Journal Article
• Authors: Rocchi, Silvia; Caretti, Fulvia; Gentili, Alessandra; Curini, Roberta; Perret, Daniela; Pérez-Fernández, Virginia
• Is Part Of: Food chemistry. Volume 211(2016)
• Page Start: 455
• Page End: 464
• Publication Date: 2016-11-15
• Subjects: Vitamin A -- Retinoids -- Retinyl esters -- Milk -- HPLC-MS -- Vitamin A vitamers profiling; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.05.066
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: The C18 /C30 column system, assembled in this work, has been able to separate sixteen retinoids. A reliable LC-DAD-MS method for profiling retinoids in real matrices has been developed. The retinoid composition of milk from different ruminants species has been elucidated. For the first time a detailed profile of retinoids in buffalo's and ewe's milk is provided. After minor changes the method could be applied to characterize retinoids in bio-clinical samples. Abstract: An effective high performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLC-DAD-MS/MS) analytical approach was developed for retinoid profiling in raw milk samples (cow, buffalo, ewe, and goat). The analytes were isolated by means of liquid-liquid extraction, including a "lipid freezing" step, with yields exceeding 66%. Since the positive atmospheric pressure chemical ionisation mass spectrometry (APCI-MS) detection is not completely selective, a reliable identification has been accomplished by fully separating the analytes on a tandem C18 /C30 column system under non-aqueous reversed phase (NARP) chromatography conditions. After validation, different milk varieties obtained from pasture-fed animals were analysed, providing, for the first time, the retinoid composition of both buffalo's and ewe's milk. According to the literature, retinyl palmitate has been found to be the most abundant vitamin A vitamer, but retinyl oleate is the prevalent form in the caprine milk.