Development of a LAMP marker of the Puroindoline a-D1b allele in bread wheat. (May 2016)

• Record Type: Journal Article
• Authors: Fukuta, Shiro; Tsuji, Takako; Suzuki, Ryoji; Matsumoto, Yuho; Ito, Kouji; Kataoka, Kouji; Kawara, Satomi; Miyake, Noriyuki
• Is Part Of: Journal of cereal science. Volume 69(2016)
• Page Start: 264
• Page End: 266
• Publication Date: 2016-05
• Subjects: Wheat -- Puroindoline -- Loop-mediated isothermal amplification -- DNA marker; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2016.03.014
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: Grain hardness of wheat ( Triticum aestivum ) is one of the most important characteristics for end-use properties. Grain texture is controlled by major locus Puroindoline gene. A large deletion of the majority of Puroindoline a ( Pina ) coding region cause hard texture. In this study, we report the development of a loop-mediated isothermal amplification (LAMP) reaction to identify Pina-D1b allele, which is popular genotype for hard type of bread wheat in Japan. In the LAMP method, Pina-D1b allele was easily detected using the simple NaOH extraction method and visualization of DNA amplification by hydroxynaphthol blue. Highlights: The LAMP primer set identified Pina-D1b gene specifically. . Simply extracted DNA could be used for the LAMP assay. . The LAMP method would be a useful tool for rapid and accurate selection for Pina-D1b allele.