Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM‐Cre mice in myeloid cell research. Issue 6 (24th April 2016)

• Record Type: Journal Article
• Authors: Orthgiess, Johannes; Gericke, Martin; Immig, Kerstin; Schulz, Angela; Hirrlinger, Johannes; Bechmann, Ingo; Eilers, Jens
• Is Part Of: European journal of immunology. Volume 46:Issue 6(2016)
• Page Start: 1529
• Page End: 1532
• Publication Date: 2016-04-24
• Subjects: Brain -- LysM‐Cre -- Lyz2 -- Microglia -- Neuroinflammation -- Neurons; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201546108
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract : To characterize LysM‐Cre mediated gene targeting in mice, we crossed LysM‐Cre mice to two independent reporter‐mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM‐Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM‐Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered.