Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM‐Cre mice in myeloid cell research. Issue 6 (24th April 2016) - Orthgiess, Johannes
Abstract : To characterize LysM‐Cre mediated gene targeting in mice, we crossed LysM‐Cre mice to two independent reporter‐mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM‐Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM‐Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered.