Development of dual-emission ratiometric probe-based on fluorescent silica nanoparticle and CdTe quantum dots for determination of glucose in beverages and human body fluids. (1st August 2016)

• Record Type: Journal Article
• Authors: Zhai, Hong; Feng, Ting; Dong, Lingyu; Wang, Liyun; Wang, Xiangfeng; Liu, Hailing; Liu, Yuan; Chen, Luan; Xie, MengXia
• Is Part Of: Food chemistry. Volume 204(2016)
• Page Start: 444
• Page End: 452
• Publication Date: 2016-08-01
• Subjects: Dual-emission ratiometric fluorescence probe -- Silica nanoparticles -- FITC -- Quantum dots -- Glucose -- Beverage; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.02.159
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Dual emission ratiometric fluorescence probe for detection of glucose was developed. The developed probe can be utilized to quantitatively and visually detect glucose. The probe was applied to detect glucose levels of beverages and biological samples. Abstract: A novel dual emission ratiometric fluorescence probe for determination of glucose has been developed. The reference dye fluorescence isothiocyanate (FITC) has been encapsulated in the silica nanoparticles and then the red emission CdTe QDs were grafted on the surface of the silica particles to obtain the fluorescence probe. With glucose and dopamine as substrates, the glucose level was proportional to the fluorescence ratio change of above probe caused by dopamine oxidation, which was produced via bienzyme catalysis (glucose oxidase and horseradish peroxidase). The established approach was sensitive and selective, and has been applied to determine the glucose in beverage, urine and serum samples. The average recoveries of the glucose at various spiking levels ranged from 95.5% to 108.9% with relative standard deviations from 1.5% to 4.3%. The results provided a clue to develop sensors for rapid determination of the target analytes from complex matrices.