Wine pomace seasoning attenuates hyperglycaemia-induced endothelial dysfunction and oxidative damage in endothelial cells. (April 2016)

• Record Type: Journal Article
• Authors: Del Pino-García, Raquel; Gerardi, Gisela; Rivero-Pérez, María D.; González-SanJosé, María L.; García-Lomillo, Javier; Muñiz, Pilar
• Is Part Of: Journal of functional foods. Volume 22(2016)
• Page Start: 431
• Page End: 445
• Publication Date: 2016-04
• Subjects: ABTS 2, 2′-azinobis 3-ethylbenzothiazoline-6-sulphonic acid -- ACE angiotensin I-converting enzyme -- Ang angiotensin -- AREs antioxidant responsive elements -- BCF potentially bioavailable fraction obtained after simulated colonic fermentation -- BGID potentially bioavailable fraction obtained after simulated gastrointestinal digestion -- CGs carbonyl groups -- eNOS endothelial nitric oxide synthase -- FC Folin–Ciocalteu -- GAE gallic acid equivalents -- GL glucose -- GSH glutathione reduced -- GSSG glutathione oxidised -- HO haem oxygenase -- HUVECs human umbilical vein endothelial cells -- LDH lactate dehydrogenase -- MDA malondialdehyde -- mDP mean degree of polymerisation -- NAD(P)H β-nicotinamide adenine dinucleotide phosphate -- NF-κB nuclear factor-kappa B -- NO nitric oxide -- NOX NAD(P)H oxidase -- Nrf2 nuclear factor-erythroid 2-related factor 2 -- Q- QUENCHER -- RAAS renin-angiotensin aldosterone system -- ROS reactive oxygen species -- SOD superoxide dismutase -- TE Trolox equivalents; Colonic metabolites -- Dietary antioxidants -- Endothelial function -- Hyperglycaemia -- Redox signalling -- Wine pomace; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.02.001
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: HUVECs treated with in vitro digested products from a seed-free wine pomace seasoning. Lower oxidative damage and endothelial dysfunction in hyperglycaemic treated cells. Observed benefits partly mediated by HO-1, NOX4 and SOD2 gene expression modulation. Digested products decreased ACE activity in vitro and ex vivo in hyperglycaemic cells. Key role of (poly)phenol colonic metabolism in the observed endothelial protection. Abstract: Dietary antioxidants such as phenolic phytochemicals may prevent/improve endothelial function impairment observed in cardiovascular diseases. This study investigates the specific protective effects of a vegetal seasoning obtained from seedless wine pomace against hyperglycaemia-induced oxidative damage and dysfunction in human umbilical vein endothelial cells EA.hy926. Non-cytotoxic doses of the bioactive compounds obtained following in vitro digestion of the seasoning were used as treatments. Digested compounds, especially colonic bacterial metabolites, restored a more balanced redox environment, prevented lipid peroxidation and cell membrane damage, ameliorated protein oxidation, and improved the balance between endothelial reactive oxygen species and nitric oxide production in hyperglycaemic cells. Reduction of angiotensin I-converting enzyme activity and gene modulation of superoxide dismutase, haem oxygenase-1, β-nicotinamide adenine dinucleotide phosphate oxidase-4, and endothelial nitric oxide synthase are proposed as the mechanisms underlying this protection. These results support the potential benefits of functional wine pomace seasonings in vascular complications associated with oxidative stress.