Application of pH-zone refining hydrostatic countercurrent chromatography (hCCC) for the recovery of antioxidant phenolics and the isolation of alkaloids from Siberian barberry herb. (15th July 2016)

• Record Type: Journal Article
• Authors: Kukula-Koch, Wirginia; Koch, Wojciech; Angelis, Apostolis; Halabalaki, Maria; Aligiannis, Nektarios
• Is Part Of: Food chemistry. Volume 203(2016)
• Page Start: 394
• Page End: 401
• Publication Date: 2016-07-15
• Subjects: Caffeic acid (PubChem CID: 689043) -- Chlorogenic acid (PubChem CID: 1794427) -- Ferulic acid (PubChem CID: 445858) -- Gallic acid (PubChem CID: 370) -- Proocatechuic acid (PubChem CID: 8468) -- p-Coumaric acid (PubChem CID: 637542) -- Sinapinic acid (PubChem CID: 637775) -- Syringic acid (PubChem CID: 10742) -- Apigenin (PubChem CID: 5280443) -- Luteolin (PubChem CID: 5280637) -- Berberine (PubChem CID: 2353) -- Magnoflorine (PubChem CID: 73337); pH-zone refining hydrostatic counter current chromatography -- Berberis sibirica -- Berberidaceae -- Antioxidant activity -- UHPLC–DAD-ESI-HRMS -- Orbitral trap -- Centrifugal partition chromatography; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.02.096
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Berberis sibirica was found to exhibit antioxidant potential. Methanol and acetone:water (70:30 V/V ) extracted the highest quant. of phenolics. pH-zone HCCC chromatography in MtBE–H2 O (1:1) purified phenolics from alkaloids. UPLC–DAD-ESI-IT-Orbitrap technique identified 8 phenolic acids and 2 flavonoids. Magnoflorine was identified in the extracts of B. sibirica for the first time. Abstract: The development of a fast hCCC method tailored to recover phenolics of Siberian barberry ( Berberis sibirica, Berberidaceae) responsible for the observed strong antioxidant activity was performed. Initially, the optimization of extraction procedure was evaluated based on the antiradical potential assessment (DPPH and Folin–Ciocalteu assays). 100 °C methanol ASE extract exhibited the highest antiradical activity (IC50 = 60 ± 4 μg/mL), and a significant TPC (159 ± 2 mg GAE/g). Thorough determination of phenolic content by UHPLC–DAD-ESI(−)HRMS revealed the presence of 10 phenolics as major constituents, and several groups of alkaloids. pH-zone refining hCCC was chosen as the most promising method for the extract's fractionation due to the ionizable character of its constituents. For this purpose a MtBE–H2 O (1:1) system with 10 mM TEA and HCl was applied leading to a phenolic fraction, free of alkaloids, with higher antioxidant capacity (IC50 = 25 μg/mL, TPC = 178 mg GAE/g). Additionally, fractionation of alkaloids was achieved resulting isolation of pharmacologically important alkaloids: magnoflorine and berberine.