Coffee consumption rapidly reduces background DNA strand breaks in healthy humans: Results of a short‐term repeated uptake intervention study. Issue 3 (29th December 2015)

• Record Type: Journal Article
• Authors: Bakuradze, Tamara; Lang, Roman; Hofmann, Thomas; Schipp, Dorothea; Galan, Jens; Eisenbrand, Gerhard; Richling, Elke
• Is Part Of: Molecular nutrition & food research. Volume 60:Issue 3(2016)
• Page Start: 682
• Page End: 686
• Publication Date: 2015-12-29
• Subjects: Coffee -- Comet assay -- DNA -- Short‐term -- Strand breaks; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201500668
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : The long‐term consumption of coffee is associated with improved DNA integrity in healthy volunteers. To test whether these effects could be shown after acute and repeated coffee consumption within a day, a short‐term study was performed. The results demonstrate that the intake of coffee within hours significantly reduced background DNA strand breaks, measured by comet assay. Abstract : Scope: Intervention studies provide evidence that long‐term coffee consumption correlates with reduced DNA background damage in healthy volunteers. Here, we report on short‐term kinetics of this effect, showing a rapid onset after normal coffee intake. Methods and results: In a short‐term human intervention study, we determined the effects of coffee intake on DNA integrity during 8 h. Healthy male subjects ingested coffee in 200 mL aliquots every second hour up to a total volume of 800 mL. Blood samples were taken at baseline, immediately before the first coffee intake and subsequently every 2 h, prior to the respective coffee intake. DNA integrity was assayed by the comet assay. The results show a significant ( p < 0.05) reduction of background DNA strand breaks already 2 h after the first coffee intake. Continued coffee intake was associated with further decrements in background DNA damage within the 8 h intervention ( p < 0.01 and p < 0.001, respectively). Mean tail intensities (TIs%) decreased from 0.33 TI% (baseline, 0 h) to 0.22 TI% (within 8 h coffee consumption). Conclusion: Repeated coffee consumption was associated with reduced background DNA strand breakage, clearly measurable as early as 2 h after first intake resulting in a cumulative overall reduction by about one‐third of the baseline value.