Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase. Issue 1 (8th October 2015)

• Record Type: Journal Article
• Authors: Guimarães, Rafaela; Calhelha, Ricardo C.; Froufe, Hugo J. C.; Abreu, Rui M. V.; Carvalho, Ana Maria; Queiroz, Maria João, R. P.; Ferreira, Isabel C. F. R.
• Is Part Of: Food & function. Volume 7:Issue 1(2016)
• Page Start: 79
• Page End: 83
• Publication Date: 2015-10-08
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c5fo00586h
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Wild Roman chamomile extracts and phenolic compounds showed capacity to inhibit the VEGFR-2 phosphorylation. Abstract : Angiogenesis is a process by which new blood vessels are formed from the pre-existing vasculature, and it is a key process that leads to tumour development. Some studies have recognized phenolic compounds as chemopreventive agents; flavonoids, in particular, seem to suppress the growth of tumor cells modifying the cell cycle. Herein, the antiangiogenic activity of Roman chamomile ( Chamaemelum nobile L.) extracts (methanolic extract and infusion) and the main phenolic compounds present (apigenin, apigenin-7- O -glucoside, caffeic acid, chlorogenic acid, luteolin, and luteolin-7- O -glucoside) was evaluated through enzymatic assays using the tyrosine kinase intracellular domain of the Vascular Endothelium Growth Factor Receptor-2 (VEGFR-2), which is a transmembrane receptor expressed fundamentally in endothelial cells involved in angiogenesis, and molecular modelling studies. The methanolic extract showed a lower IC50 value (concentration that provided 50% of VEGFR-2 inhibition) than the infusion, 269 and 301 μg mL −1, respectively. Regarding phenolic compounds, luteolin and apigenin showed the highest capacity to inhibit the phosphorylation of VEGFR-2, leading us to believe that these compounds are involved in the activity revealed by the methanolic extract.