Isolation, structural characterization, and antioxidativity of polysaccharide LBLP5-A from Lycium barbarum leaves. Issue 2 (February 2016)

• Record Type: Journal Article
• Authors: Gong, Guiping; Fan, Jiangbo; Sun, Yujiao; Wu, Yanmin; Liu, Yang; Sun, Wei; Zhang, Ying; Wang, Zhongfu
• Is Part Of: Process biochemistry. Volume 51:Issue 2(2016:Feb.)
• Page Start: 314
• Page End: 324
• Publication Date: 2016-02
• Subjects: Lycium barbarum leaves -- Polysaccharide -- Structure -- Antioxidativity; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2015.11.013
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: A water-soluble polysaccharide, termed as LBLP5-A, was isolated from Lycium barbarum leaves, which is a well-known medical drug in Asia. Detailed structure patterns and anti-oxidant activity of LBLP5-A-OL1 were characterized. Highlights: A novel polysaccharide (LBLP5-A) was purified from Lycium barbarum leaves. The structure of LBLP5-A was determined by several chemical methods combined with electrospray ionization–mass spectrometry (ESI–MS). LBLP5-A exhibited a good scavenging ability on superoxide, hydroxyl, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Abstract: A water-soluble polysaccharide, LBLP5-A, was isolated from Lycium barbarum leaves, which is widely used as a medicine in Asia. The average molecular weight of LBLP5-A is 11.33 × 10 4 Da. With O-type carbohydrate–peptide linkage in its structure, LBLP5-A afforded glycan fractions of LBLP5-A-OL1, LBLP5-A-OL2, LBLP5-A-OL3, and LBLP5-A-OL4 acquired by β-elimination reaction. Detailed structure patterns of LBLP5-A-OL1 were characterized by monosaccharide composition analysis, methylation analysis, partial acid hydrolysis, Smith degradation, one- or two-dimensional (1D/2D) nuclear magnetic resonance (NMR), and electrospray ionization–mass spectrometry (ESI–MS). LBLP5-A-OL1 was identified to be a highly branched polysaccharide with a backbone of (1 → 3)-linked Gal, which was partially substituted at its O-6 position. These branches, with arabinose and galactose terminals, were assigned to be (1 → 3)-linked Gal, (1 → 4)-linked Gal, (1 → 3)-linked Ara f, (1 → 5)-linked Ara f, and (1 → 2, 4)-linked Rha f. LBLP5-A-OL3 and LBLP5-A-OL4 were determined to be a series of oligosaccharides that share the general structure pattern of Aran1 → Galn2 → Rhan3 ( n 1 = 0–6, n 2 = 0–8, n 3 = 0–1) by ESI–MS. Moreover, LBLP5-A exhibited excellent antioxidativity, concerning the scavenging abilities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radicals in vitro .