Synthesis of rebaudioside-A by enzymatic transglycosylation of stevioside present in the leaves of Stevia rebaudiana Bertoni. (1st June 2016)

• Record Type: Journal Article
• Authors: Adari, Bhaskar Rao; Alavala, Sateesh; George, Sara A.; Meshram, Harshadas M.; Tiwari, Ashok K.; Sarma, Akella V.S.
• Is Part Of: Food chemistry. Volume 200(2016)
• Page Start: 154
• Page End: 158
• Publication Date: 2016-06-01
• Subjects: Stevioside (PubChem CID:442089) -- Rebaudioside-A (PubChem CID:6918840); Stevia leaves -- Transglycosylation -- Cellulase -- Glucosyl donors -- Rebaudioside-A; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.01.033
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Enzyme mediated transglycosylation of stevioside to rebaudioside-A. The glucosyl moiety transglycosylated stereospecifically to get 13-O-β-stevioside. The rebaudioside-A content in the pre-treated stevia leaves increased from 4% to 66%. 95% pure rebaudioside-A was obtained by multiple column chromatography. Abstract: Rebaudioside-A is the second most abundant sweet diterpene glycoside (1–3%) present in the leaves of Stevia rebaudiana Bertoni, and is now being considered as a possible sucrose substitute due to its pleasant organoleptic properties and associated health benefits. In the present study, a novel in situ enzymatic transglycosylation of stevioside has been developed by pre-treating the stevia leaves with cellulase and adding soluble starch as the glucosyl donor. The results confirm that the transglycosylation of stevioside led to an enrichment in the rebaudioside-A content from 4% to 66%. This was further purified by multiple column chromatography to obtain 95% pure rebaudioside-A. The isolated rebaudioside-A showed concentration-dependent α-glucosidase inhibitory activity with IC50 = 35.01 μg/ml. Thus the study highlights the biotransformation of stevioside present in stevia leaves to rebaudioside-A by a simple, inexpensive and eco-friendly process that has commercial potential.