Preservation of anthocyanins in solid lipid nanoparticles: Optimization of a microemulsion dilution method using the Placket–Burman and Box–Behnken designs. (15th May 2016)

• Record Type: Journal Article
• Authors: Ravanfar, Raheleh; Tamaddon, Ali Mohammad; Niakousari, Mehrdad; Moein, Mahmoud Reza
• Is Part Of: Food chemistry. Volume 199(2016)
• Page Start: 573
• Page End: 580
• Publication Date: 2016-05-15
• Subjects: Solid lipid nanoparticles -- Microemulsion dilution -- Red cabbage -- Anthocyanins; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.12.061
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Anthocyanin from red cabbage were loaded in SLNs by microemulsion dilution method. SLN was able to encapsulate and preserve anthocyanins from degradation. Anthocyanins were encapsulated at high entrapment efficiency. The microencapsulation increased the stability of the anthocyanins against high pH. Abstract: Anthocyanins are the main polyphenol components from red cabbage ( Brassica oleracea L. Var. Capitata f. Rubra) extracts that have inherent antioxidant activities. Anthocyanins are effectively stable in acidic gastric digestion conditions, with nearly 100% phenol content recovery. However, the total phenol content recovery after simulated pancreatic digestion was approximately 25%. To protect anthocyanins against harsh environmental conditions (e.g., pH and temperature), solid lipid nanoparticles were prepared by the dilution of water in oil (w/o) microemulsions containing anthocyanins in aqueous media. The formulations were characterized for particle size and encapsulation efficiency. The formulation parameters (e.g., volume of the internal aqueous phase, homogenization time and the percentages of total lipid, total surfactant or stabilizer) were optimized using the Placket–Burman and Box–Behnken experimental designs. Entrapment efficiency (89.2 ± 0.3%) was calculated when the mean particle size was 455 ± 2 nm. A scanning electron microscopy study revealed the spherical morphology of the particles.