Analysis of urinary TGF-β1, MCP-1, NGAL, and IL-17 as biomarkers for lupus nephritis. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Susianti, Hani; Iriane, Vincentia M.; Dharmanata, Suriya; Handono, Kusworini; Widijanti, Anik; Gunawan, Atma; Kalim, Handono
• Is Part Of: Pathophysiology. Volume 22:Issue 1(2015)
• Page Start: 65
• Page End: 71
• Publication Date: 2015-03
• Subjects: Lupus nephritis -- uTGF-β1 -- uMCP-1 -- uNGAL -- uIL-17; Physiology, Pathological -- Periodicals; 571.9
• DOI: 10.1016/j.pathophys.2014.12.003
• Languages: English
• ISSNs: 0928-4680

Abstract

Abstract: This study was aimed to determine the diagnostic performance of transforming growth factor beta 1 (uTGF-β1), monocyte chemoattractant protein-1 (uMCP-1), neutrophil gelatinase-associated lipocalin (uNGAL) and interleukin 17 (uIL-17) in LN. Seventy participants were studied, categorized into three groups: 38 severe LN (class III–IV LN patients); 12 mild LN (class I–II LN patients); and 20 control (healthy volunteers). Diagnosis of SLE was based on the 1997 ARA criteria. Class NL classified according to ISN/RPS 2004. uTGF-β1, uMCP-1, uNGAL, uIL-17 levels were determined by ELISA, using spot urine. The level of uMCP-1 and uNGAL was significantly greater in severe or mild LN compared with control group ( P < 0.05). The level of uTGF-β1 and uIL-17 was significantly higher in severe LN than that controls group ( P < 0.05). The AUC of uTGF-β1, uMCP-1, uNGAL, uIL-17 was 66.50%; 86.90%; 87.50%; 71.70%, with the cut-off value of 27.13 pg/ml; 1.54 pg/ml; 446.30 pg/ml; 36.62 pg/ml. Only uNGAL showed a significant correlation with the activity ( P = 0.016; r = 0.389) and chronicity indices ( P = 0.018; r = 0.381). This study showed that uTGF-β1, uMCP-1, uNGAL, uIL-17 levels were increased in LN. The AUC values for each biomarker are indicating a good diagnostic value. Urinary NGAL had the best sensitivity and specificity followed by uMCP-1, uIL-17 and uTGF-β1. For combinations of two biomarkers, the best sensitivity and specificity were displayed by the combination of uTGF-β1 & u-NGAL, followed by uMCP-1 & uNGAL.