Studies on anti-inflammatory activity of sulfated polysaccharides from cultivated fungi Antrodia cinnamomea. (February 2016)

• Record Type: Journal Article
• Authors: Cheng, Jing-Jy; Chao, Chi-Hsein; Chang, Pin-Chun; Lu, Mei-Kuang
• Is Part Of: Food hydrocolloids. Volume 53(2015:Nov.)
• Page Start: 37
• Page End: 45
• Publication Date: 2016-02
• Subjects: Antrodia cinnamomea -- Polysaccharides -- Sulfated polysaccharides -- Molecular-weight-distribution -- Anti-Inflammation; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2014.09.035
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: Polysaccharides (PSs) and sulfated polysaccharides (SPSs) were isolated from sodium sulfate-, and potassium sulfate-feeding mycelia of Antrodia cinnamomea and their chemical components and molecular weight distribution were studied. The highest value of SPS content in the SPSs was 1.22 mmol/g SPSs for sodium sulfate 1 mM feeding for 49 days. SPSs of A. cinnamomea contained five fractions. The area percentage of low-molecular-weight SPSs (<20 kDa) covered almost half of the SPSs mixture. Glucose was the major sugar in the SPS mixture. To study the effects of SPSs on anti-inflammation activity, an in vitro assay was performed on lipopolysaccharide (LPS)-stimulated inflammation in RAW264.7 macrophages. SPSs showed a stronger inhibitory effect on tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) release compared to those of non-sulfated polysaccharide (PS), with both at values of 100% suppression. This fact suggests that the sulfation degree of SPS may play a role in anti-inflammatory activity. Graphical abstract: Highlights: Sulfated polysaccharides (SPSs) were prepared from mycelia of Antrodia cinnamomea . Glucose was the major sugar of SPSs. Low-molecular-weight SPSs (<20 kDa) covered almost half of the SPSs mixture. SPSs showed inhibitory effect on tumor necrosis factor-α and interleukin-6 release. Sulfation degree of SPS may play a role in anti-inflammatory activity.