Hypoglycemic and hepatoprotective effects of d-chiro-inositol-enriched tartary buckwheat extract in high fructose-fed mice. Issue 12 (28th September 2015)

• Record Type: Journal Article
• Authors: Hu, Yuanyuan; Zhao, Yan; Ren, Daoyuan; Guo, Jianjun; Luo, Yiyang; Yang, Xingbin
• Is Part Of: Food & function. Volume 6:Issue 12(2015)
• Page Start: 3760
• Page End: 3769
• Publication Date: 2015-09-28
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c5fo00612k
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : d - Chiro -Inositol-Enriched Tartary Buckwheat Extract (DTBE) prevents high fructose-induced hyperglycemia and hepatic injury in mice. Abstract : This study was designed to investigate the protective effects ofd - Chiro -Inositol (DCI) enriched tartary buckwheat extract (DTBE) against high fructose (HF) diet-induced hyperglycemia and liver injury in mice. HPLC analysis revealed that the content of DCI present in purified DTBE was 34.06%. Mice fed 20% fructose in drinking water for 8 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia, hepatic steatosis and oxidative stress ( p < 0.01). Continuous administration of DTBE in HF-fed mice dose-dependently reduced the HF-induced elevation of body weight, serum glucose, insulin, total cholesterol (TC), total triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) levels, as well as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), C-reactive protein (CRP) and lactate dehydrogenase (LDH) activities, while the HF-induced decline of serum high density lipoprotein-cholesterol (HDL-C) levels could be markedly elevated in the mice. Meanwhile, DTBE also dose-dependently increased the hepatic total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities, and decreased hepatic malonaldehyde (MDA) levels, relative to HF-treated mice. Histopathology of H&E and Oil Red O staining confirmed liver injury induced by a HF diet and the hepatoprotective effect of DTBE. These findings are the first to demonstrate that the intake of DTBE may be a feasible preventive or therapeutic strategy for HF diet-induced hyperglycemia, hepatic steatosis and oxidative injury.