Optimisation of α‐terpineol production by limonene biotransformation using Penicillium digitatum DSM 62840. (9th April 2015)

• Record Type: Journal Article
• Authors: Tai, Ya‐Nan; Xu, Min; Ren, Jing‐Nan; Dong, Man; Yang, Zi‐Yu; Pan, Si‐yi; Fan, Gang
• Is Part Of: Journal of the science of food and agriculture. Volume 96:Number 3(2016)
• Page Start: 954
• Page End: 961
• Publication Date: 2015-04-09
• Subjects: biotransformation -- Penicillium digitatum -- monoterpene -- (R)‐(+)‐limonene -- α‐terpineol; Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.7171
• Languages: English
• ISSNs: 0022-5142

Abstract

Abstract: BACKGROUND: In this study, ( R )‐(+)‐limonene biotransformation using three fungal strains was compared. Penicillium digitatum DSM 62840 was distinguished for its capacity to transform limonene into α ‐terpineol with high regioselectivity. Growth kinetics in submerged liquid culture and the effects of growth phase and contact time on biotransformation were studied using this strain. Substrate concentration, co‐solvent selection, and cultivation conditions were subsequently optimised. RESULTS: The maximum concentration of α ‐terpineol (833.93 mg L −1 ) was obtained when the pre‐culture medium was in medium log‐phase by adding 840 mg L −1 substrate dissolved in ethanol and cultivation was performed at 24 °C, 150 rpm, and pH 6.0 for 12 h. Addition of small amounts of ( R )‐(+)‐limonene (84 mg L −1 ) at the start of fungal log‐phase growth yielded a 1.5‐fold yield of α ‐terpineol, indicating that the enzyme was inducible. CONCLUSION: Among these three strains tested, P. digitatum DSM 62840 was proved to be an efficient biocatalyst to transform ( R )‐(+)‐limonene to α ‐terpineol. Further studies revealed that the optimal growth phase for biotransformation was in the medium log phase of this strain. The biotransformation represented a wide tolerance of temperature; α ‐terpineol concentration underwent no significant change at 8–32 °C. The biotransformation could also be performed using resting cells. © 2015 Society of Chemical Industry