A combination of solid‐phase extraction and dispersive solid‐phase extraction effectively reduces the matrix interference in liquid chromatography–ultraviolet detection during pyraclostrobin analysis in perilla leaves. (23rd June 2015)

• Record Type: Journal Article
• Authors: Farha, Waziha; Rahman, Md. Musfiqur; Abd El‐Aty, A. M.; Jung, Da‐I.; Kabir, Md. Humayun; Choi, Jeong‐Heui; Kim, Sung‐Woo; Jeong Im, So; Lee, Young‐Jun; Shin, Ho‐Chul; Kwon, Chan‐Hyeok; Son, Young‐Wook; Lee, Kang‐Bong; Shim, Jae‐Han
• Is Part Of: Biomedical chromatography. Volume 29:Number 12(2015:Dec.)
• Page Start: 1932
• Page End: 1936
• Publication Date: 2015-06-23
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3523
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid‐phase extraction and dispersive solid‐phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (<italic>R²</italic> = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06– 89.10% of the target compound was recovered with a relative standard deviation &lt;4. The limits of detection and quantification were 0.0033 and 0.01 mg/kg, respectively. The method was successfully applied to field samples collected from two different areas at Gwangju and Muan. The decline in the resiudue concentrations was best ascribed to a first‐order kinetic model with half‐lives of 5.7 and 4.6 days. The variation between the patterns was attributed to humidity. Copyright © 2015 John Wiley &amp; Sons, Ltd.</p> </abstract>