A combination of solid‐phase extraction and dispersive solid‐phase extraction effectively reduces the matrix interference in liquid chromatography–ultraviolet detection during pyraclostrobin analysis in perilla leaves. (23rd June 2015) - Farha, Waziha
Abstract Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid‐phase extraction and dispersive solid‐phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (R2 = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06– 89.10% of the target compound was recovered with a relative standard deviation <4. The limits of detection and quantification were 0.0033 and 0.01 mg/kg, respectively. The method was successfully applied to field samples collected from two different areas at Gwangju and Muan. The decline in the resiudue concentrations was best ascribed to a first‐order kinetic model with half‐lives of 5.7 and 4.6 days. The variation between the patterns was attributed to humidity. Copyright © 2015 John Wiley & Sons, Ltd.