Growth Inhibition and Cytotoxicity in Human Lung and Cervical Cancer Cell Lines and Glutathione S‐Transferase Inhibitory Activity of Selected Sri Lankan Traditional Red Rice (Oryza Sativa L.) Brans. Issue 5 (29th June 2015)

• Record Type: Journal Article
• Authors: Abeysekera, W.K.S.M.; Premakumara, G.A.S.; Dar, Ahsana; Choudhary, M. Iqbal; Ratnasooriya, W.D.; Kashif, Muhammad; Mudassar, C.; Ali, S.R.; Chandrasekharan, N.V.
• Is Part Of: Journal of food biochemistry. Volume 39:Issue 5(2015:Oct.)
• Page Start: 585
• Page End: 593
• Publication Date: 2015-06-29
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.12162
• Languages: English
• ISSNs: 0145-8884

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jfbc12162-sec-0001" sec-type="section"> <p>Extracts, fractions and gastrointestinal‐resistant protein hydrolysates (GRPH) from rice bran (RB) of four Sri Lankan traditional varieties were studied for growth inhibition (GI) and cytotoxicity against human lung cancer (NCI‐H460), cervical cancer (HeLa) cell lines and effect on glutathione S‐transferase (GST) <italic>in vitro</italic>. RB extracts showed significantly high (<italic>P</italic> < 0.05) GI (GI<sub>50</sub> μg/mL: HeLa: 19.24 ± 3.47 to 112.58 ± 0.05; NCI‐H460: 54.63 ± 4.89 to 170.31 ± 4.71) and cytotoxicity (LC<sub>50</sub> μg/mL: HeLa: 240.12 ± 9.23 to 476.22 ± 0.05; NCI‐H460: 255.36 ± 1.81 to 412.07 ± 17.73) compared with GRPH. Highest GI (GI<sub>50</sub>: 30.63 ± 9.19 to 44.40 ± 5.34 μg/mL) and cytotoxicity (LC<sub>50</sub>: 141.31 ± 14.47 to 169.51 ± 2.04 μg/mL) was evident in nonpolar fractions (dichloromethane: Goda Heeneti and Sudu Heeneti; hexane: Dik Wee and Sudu Heeneti). No cytotoxicity was evident to normal cells. All RB extracts had high GST inhibition. Properties observed indicate its usefulness in managing lung and cervical cancers.</p> </sec> <sec id="jfbc12162-sec-0002" sec-type="section"> <title>Practical Applications</title> <p>Lung and cervical cancers are two of the most commonly diagnosed cancers in men and women, respectively, and leading causes of mortality. Although numerous potent chemotherapeutic drugs are available for management, most of these drugs are very expensive and are associated with numerous side effects. Further, anticancer drugs containing electrophilic centers can easily form a conjugate with glutathione, which is facilitated by glutathione S‐transferase (GST) and excreted from the body resulting in lower efficacy. Therefore, GST inhibitors are used as adjuvants during cancer chemotherapy. The selected rice varieties in this study claimed to possess health‐enhancing properties according to Sri Lankan ethnomedicine. The findings of this study clearly showed that bran extracts, fractions and gastrointestinal‐resistant protein hydrolysates of selected rice varieties had growth inhibition and cytotoxicity against both lung and cervical cancers. Further, all of these selected varieties had high GST inhibition. Therefore, selected rice brans may be useful in the management of lung and cervical cancers.</p> </sec> </abstract>