High yield ultrasonication extraction method for Undaria pinnatifida sporophyll and its anti-inflammatory properties associated with AP-1 pathway suppression. Issue 2 (December 2015)

• Record Type: Journal Article
• Authors: Song, Kyung-Mo; Ha, Su Jong; Lee, Jang-Eun; Kim, Soon-Hee; Kim, Yong Ho; Kim, Youngeon; Hong, Sang Pil; Jung, Sung Keun; Lee, Nam Hyouck
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 64:Issue 2(2015:Dec.)
• Page Start: 1315
• Page End: 1322
• Publication Date: 2015-12
• Subjects: Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2015.07.055
• Languages: English
• ISSNs: 0023-6438

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Developing efficient and eco-friendly extraction methods is a major goal of the functional food industry. We developed an ultrasonication extraction method for <italic>Undaria pinnatifida</italic> sporophyll requiring 6 h extraction time at 80% amplitude. This application of ultrasonication increases yield by 25% and decreases extraction time by approximately 18 h. Additionally, we investigated the effect of the ultrasonicated sporophyll from <italic>Undaria pinnatifida</italic> extract (UPE) on LPS-induced inflammation. Following ultrasonication, the average molecular weight decreases in comparison to conventional methods, in a similar manner to commercial fucoidan. UPE significantly suppresses lipopolysaccharide (LPS)-induced nitrite and PGE<sub>2</sub> production and subsequently suppresses inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression in Raw 264.7 cells. Western blot assay results also demonstrate that UPE strongly suppresses LPS-induced c-Jun N terminal kinase (JNK) 1/2/MKK 4 and p38/MKK3/6 phosphorylation in Raw 264.7 cells. SR11302, an AP-1 specific inhibitor, suppressed LPS-induced iNOS and COX-2 expression. In addition, SiMLK3 strongly suppressed LPS-induced iNOS and COX-2 expression, as well as JNK1/2 and p38 phosphorylation in Raw 264.7 cells.</p> <p id="abspara0015">Taken together, these results suggest that this ultrasonication extraction method is more efficient for extracting sporophyll of <italic>Undaria pinnatifida</italic> extract, which has potent anti-inflammatory properties.</p> </sec> </abstract>