Ω3‐polyunsaturated fatty acids suppress lipoprotein‐associated phospholipase A2 expression in macrophages and animal models. Issue 9 (18th June 2015)

• Record Type: Journal Article
• Authors: Li, Zhuang; Ren, Wenzhi; Han, Xiaolei; Liu, Xingxing; Wang, Gangqi; Zhang, Mingjun; Pang, Daxin; Ouyang, Hongsheng; Tang, Xiaochun
• Is Part Of: Molecular nutrition & food research. Volume 59:Issue 9(2015:Sep.)
• Page Start: 1771
• Page End: 1779
• Publication Date: 2015-06-18
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201500022
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2417-sec-0010" sec-type="section"> <title>Scope</title> <p>ω3‐polyunsaturated fatty acids (ω3‐PUFAs) have beneficial effects on cardiovascular function, and lipoprotein‐associated phospholipase A2 (Lp‐PLA<sub>2</sub>) is associated with the risk of cardiovascular disease. Here, we investigated the effects of ω3‐PUFAs on Lp‐PLA<sub>2</sub> expression in vitro and in vivo and explored the mechanisms involved.</p> </sec> <sec id="mnfr2417-sec-0020" sec-type="section"> <title>Methods and results</title> <p>Human monocyticcells (THP‐1) were induced into macrophages in an in vitro model. ω3‐PUFAs suppressed Lp‐PLA<sub>2</sub> expression; the suppression induced by docosahexaenoic acid (DHA) was related to reduced inflammation. Tumor necrosis factor‐α (TNF‐α) was employed to stimulate the phosphorylation of p38 mitogen‐activated protein kinase (MAPK), nuclear factor‐κB (NF‐κB) p65 and Lp‐PLA<sub>2</sub> expression in macrophages. The stimulation was inhibited by DHA and the anti‐inflammatory drug sodium salicylate. Moreover, the stimulation of Lp‐PLA<sub>2</sub> expression by TNF‐α could be suppressed by NF‐κB and MAPK pathway inhibitors. Then, chronic inflammation was induced in an in vivo mouse model, resulting in an increase in Lp‐PLA<sub>2</sub> expression in peripheral blood mononuclear cells (PBMCs) and arteries. This increase was suppressed by ω3‐PUFAs. Inhibition of Lp‐PLA<sub>2</sub> transcription in PBMCs was also observed in ω3‐PUFA‐enriched swine.</p> </sec> <sec id="mnfr2417-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Our results demonstrate that the protective effects of ω3‐PUFAs against cardiovascular events may be related to the suppression of Lp‐PLA<sub>2</sub> levels.</p> </sec> </abstract>