Removal of Anisakis simplex allergens from infected fish during the washing step of surimi production. (4th December 2014)

• Record Type: Journal Article
• Authors: Olivares, Fabiola; González‐Muñoz, Miguel; Carballeda‐Sangiao, Noelia; Rodríguez‐Mahillo, Ana; Careche, Mercedes; de las Heras, Cristina; Navas, Alfonso; Tejada, Margarita
• Is Part Of: Journal of the science of food and agriculture. Volume 95:Number 13(2015:Oct. 15)
• Page Start: 2626
• Page End: 2631
• Publication Date: 2014-12-04
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6994
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main" id="jsfa6994-abs-0001"> <title>Abstract</title> <sec id="jsfa6994-sec-0001" sec-type="section"> <title>BACKGOUND</title> <p id="jsfa6994-para-0001">The washing operation of fish muscle is one of the key steps in the production of <italic>surimi</italic>. The aim of this study was to assess in parasitised minced fish the effect of the washing steps on the allergen removal of <italic>Anisakis simplex</italic> and on protein yield during <italic>surimi</italic> processing. Experimentally infected hake (<italic>Merluccius merluccius</italic>) (50 <italic>Anisakis simplex s.s</italic> L3 larvae per 100 g of muscle) underwent three successive washing steps with water, phosphate buffer (20 mmol L<sup>−1</sup>), sodium bicarbonate (60 mmol L<sup>−1</sup>), or sodium hypochlorite (0.27 mmol L<sup>−1</sup>) in the <italic>surimi</italic> processing (4 kg muscle, 1:4 w/v for each solution). Total protein concentration and <italic>A. simplex</italic> antigens and allergens were evaluated in each waste fraction.</p> </sec> <sec id="jsfa6994-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jsfa6994-para-0002">The highest removal of Ani s 4 and <italic>A. simplex</italic> antigens was achieved by using phosphate buffer, together with a good protein yield in the raw <italic>surimi</italic>. Decrease of the concentration of allergens and antigens as a function of the washing steps rendered a linear trend (<italic>R</italic><sup>2</sup> = 0.95 and 0.98 for Ani s 4 and <italic>A. simplex</italic> antigens, respectively).</p> </sec> <sec id="jsfa6994-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jsfa6994-para-0003">The conditions for an optimal removal of <italic>Anisakis</italic> allergens can be established and calculated as a function of the washing steps. This approach opens a line to utilise parasitised fish in a safer way. © 2014 Society of Chemical Industry</p> </sec> </abstract>