Ascorbic acid modulates cell migration in differentiated HL‐60 cells and peripheral blood leukocytes. Issue 8 (8th May 2015)

• Record Type: Journal Article
• Authors: Schwager, Joseph; Bompard, Albine; Weber, Peter; Raederstorff, Daniel
• Is Part Of: Molecular nutrition & food research. Volume 59:Issue 8(2015:Aug.)
• Page Start: 1513
• Page End: 1523
• Publication Date: 2015-05-08
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201400893
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2388-sec-0010" sec-type="section"> <title>Scope</title> <p>The impact of L‐ascorbic acid (L‐AA) on the chemokinesis (CK) and chemotaxis (CT) of HL‐60 cells and polymorphonuclear cells (PMN) was investigated.</p> </sec> <sec id="mnfr2388-sec-0020" sec-type="section"> <title>Methods and results</title> <p>HL‐60 cells were differentiated with DMSO, retinoic acid (RA), vitamin D, or L‐AA. Chemokinesis and chemotaxis of differentiated HL‐cells were assayed. Vitamin D3‐treated HL‐60 cells (dHL‐60<sub>vitD3</sub> cells) and RA‐treated cells (dHL‐60<sub>RA</sub> cells) acquired monocyte/macrophage‐like and neutrophil‐like phenotypes, respectively. DMSO induced the differentiation of an intermediate phenotype (dHL‐60<sub>DMSO</sub> cells), whereas L‐AA downregulated neutrophil markers (dHL‐60<sub>L‐AA</sub> cells). dHL‐60<sub>DMSO</sub> cells had increased CK and potent CT in gradients of IL‐8 and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine (fMLP). dHL‐60<sub>RA</sub> cells and dHL‐60<sub>L‐AA</sub> cells migrated less toward IL‐8 and fMLP; dHL‐60<sub>vitD3</sub> cells preferably responded to fMLP. L‐AA enhanced CK of dHL‐60<sub>DMSO</sub> cells and was a weak chemo‐attractant. In human leukocytes, IL‐8 and fMLP triggered receptor‐mediated chemotaxis. CXCR2 and fMLPR were downregulated by IL‐8 and fMLP, respectively. L‐AA stimulated chemotaxis although significantly less than IL‐8 and fMLP. IL‐8 targeted chemotaxis was enhanced both in HL‐60 cells and leukocytes when cells were incubated with L‐AA.</p> </sec> <sec id="mnfr2388-sec-0030" sec-type="section"> <title>Conclusion</title> <p>L‐AA modulated chemokinesis and had significant chemo‐attractant properties, which were independent on fMLP or IL‐8 receptors. The results suggest that L‐AA improves leukocyte function in innate immune responses.</p> </sec> </abstract>