Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Zheng, Ming-Min; Wang, Ru-Feng; Li, Chun-Xiu; Xu, Jian-He
• Is Part Of: Process biochemistry. Volume 50:Issue 4(2015:Apr.)
• Page Start: 598
• Page End: 604
• Publication Date: 2015-04
• Subjects: Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2014.12.026
• Languages: English
• ISSNs: 1359-5113

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">A new 7β-HSDH gene was cloned and its enzymatic properties were characterized.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">CDCA was transformed to UDCA using two enzymes with self-recycling of cofactor.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">By a two-step reaction strategy, the UDCA yield was improved up to 98%.</p> </list-item> </list> </p> </sec> </abstract>