Cloning, expression and in silico studies of a serine protease from a marine actinomycete (Nocardiopsis sp. NCIM 5124). Issue 3 (March 2015)

• Record Type: Journal Article
• Authors: Rohamare, Sonali; Gaikwad, Sushama; Jones, Dafydd; Bhavnani, Varsha; Pal, Jayanta; Sharma, Ranu; Chatterjee, Prathit
• Is Part Of: Process biochemistry. Volume 50:Issue 3(2015:Mar.)
• Page Start: 378
• Page End: 387
• Publication Date: 2015-03
• Subjects: Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2014.12.025
• Languages: English
• ISSNs: 1359-5113

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">Serine protease from <italic>Nocardiopsis</italic> sp. cloned and expressed.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">pET-39b (+) served as better vector than pET-22b (+).</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">Homology model suggested the protein to be a member of PA clan.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Unfolding cooperativity was studied by novel computational approach.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Docking with substrate showed important binding interactions.</p> </list-item> </list> </p> </sec> </abstract>