A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents. (April 2015)

• Record Type: Journal Article
• Authors: Rodriguez, Paula; Magallanes-Noguera, Cynthia; Menéndez, Pilar; Orden, Alejandro A.; Gonzalez, David; Kurina-Sanz, Marcela; Rodríguez, Sonia
• Is Part Of: Biocatalysis and biotransformation. Volume 33:Number 2(2015)
• Page Start: 121
• Page End: 129
• Publication Date: 2015-04
• Subjects: Enzymes -- Biotechnology -- Periodicals; Enzymes -- Industrial applications -- Periodicals; Biotransformation (Metabolism) -- Periodicals; 660.63
• DOI: 10.3109/10242422.2015.1053471
• Languages: English
• ISSNs: 1024-2422

Abstract

<abstract> <title>Abstract</title> <p>The reported anti-Prelog selectivity in <italic>Raphanus sativus</italic> hairy roots prompted us to search for similar activity in other <italic>R. sativus</italic> systems, such as <italic>in vitro</italic> germinated sprouts, edible roots, and its associated endophytic microorganisms. Two test substrates were used in these studies: acetophenone and <italic>rac</italic>-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated for their ability to reduce both substrates. Among the isolates, two bacteria and a fungus having the potential to be used as biocatalysts were found: <italic>Bacillus megaterium</italic> which reduces acetophenone with excellent anti-Prelog selectivity, and <italic>Pseudomonas</italic> sp. and <italic>Penicillium chrysogenum</italic> with enantioselectivity and stereoselectivity for the reduction of <italic>rac</italic>-ethyl 2-ethyl-3-oxobutanoate, respectively.</p> </abstract>